HPLC coupled with Box–Behnken design for the simultaneous quantification of methylparaben and propylparaben in cream formulations

DOI https://doi.org/10.31130/ud-jst.2026.24(9).317E

Tóm tắt: 8 | PDF: 13

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Author

  • Hai Ngo Dang Truong
    Dong A University, Vietnam
    Hien Nguyen Thi Ngoc
    Dong A University, Vietnam
    Ho Dang Ngoc
    Dong A University, Vietnam

Từ khóa:

HPLC
methylparaben
propylparaben
Box–Behnken design
response surface methodology

Tóm tắt

The study employed high-performance liquid chromatography (HPLC) combined with Box–Behnken design (BBD), response surface methodology (RSM), and the Derringer desirability function to simultaneously quantify methylparaben (MP) and propylparaben (PP) in cream formulations. The factors investigated were mobile phase composition, column temperature, and flow rate. The responses included PP retention time, MP–PP resolution, and MP asymmetry factor. Optimal conditions were obtained at an ACN:H2O ratio of 51.7:48.3, column temperature of 40°C, and flow rate of 1.16 mL/min. The deviation between predicted and experimental values was below 6%, indicating good model reliability. The method was validated according to ICH guidelines and met requirements for linearity, accuracy, and precision. Limits of detection and quantification for both compounds were 0.01 and 0.03 μg/mL, respectively. The method was successfully applied to commercial cream samples. The results confirm the effectiveness of combining HPLC with BBD in reducing experimental runs and improving analytical performance.

Tài liệu tham khảo

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Sep 30, 2026
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Truong, H. N. D., H. N. T. Ngoc, và H. D. Ngoc. “HPLC Coupled With Box–Behnken Design for the Simultaneous Quantification of Methylparaben and Propylparaben in Cream Formulations”. Tạp Chí Khoa học Và Công nghệ Đại học Đà Nẵng, vol 24, số p.h 9, Tháng Chín 2026, tr 102-6, doi:10.31130/ud-jst.2026.24(9).317E.

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