PRODUCING ENZYME CELLULASE FROM TRICHODERMA HARZIANUM AND USING ENZYME CELLULASE TO HYDROLYZE OKARA

Abstract

In this paper, we present research results on several technological conditions for obtaining enzyme cellulase from Trichoderma harzianum and using this enzyme to hydrolyze okara based on the enzyme method. The conditions for Trichoderma harzianum to biosysthesize cellulase with high enzymic activity (4.40 IU/ml) in semi-fermentation environment with Tricoderma 5% having cell density of 7.8 x 108 cell/ml at 300C are: (i) percentage of supplemented okara 5%, (ii) initial humidity 60%, and (iii) incubation duration 120 hours. Research results on effects of enzyme/substrate ratio, duration and temperature show that the best conditions for enzymic hydrolysis are: (i) enzyme/substrate ratio 4.5/5 (v/w), (ii) duration 132 hours, and (iii) temperature 50OC. Under the above conditions, the amount of reducing sugar obtained is 5.523 g /l.
Keywords: okara, hydrolysis, biosynthesis, reducing sugar, enzymic activity.