Isolation of Stigmasterol from methanolic extract of stem and foliage of Abrus precatorius Linn.

Tóm tắt

Abrus precatorius Linn. belongs to Fabaceae family. Its aerial parts are used for treating certain health problems like leucorrhoea, gonorrhoea, diarrhoea and dysentery. The purpose of this study is to identify, isolate and determine structure of isolated compound from the stem and foliage of red form of Abrus precatorius Linn which are collected from the Dai Loc District, Quang Nam Province, Vietnam. To isolate the compound, the dried stem and foliage powder of Abrus precatorius Linn are extracted with methanol at room temperature and the solvent is recovered under low pressure to obtain crude methanol extract. Crude methanol extract is extracted again with dichloromethane and the solvent is recovered under low pressure to obtain crude dichloromethane extract. The isolated compound is purified from crude dichloromethane extract by silica gel column chromatography. The structure of the isolated compound is determined as Stigmasterol by IR, 1H-NMR, 13C-NMR and GC.