Aplication of method “StreamDrop” for chromosome preparation in Allium ascalonicum L.
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Keywords:
Chromosome
Allium ascalonicum L
StreamDrop
cellulase
mitosis
Abstract
The chromosome preparation is a basic and a key step in plant cytogenetic studies. In this paper, we present results of the research of different conditions for high quality chromosome preparation with “StreamDrop” method. The results have showen that samplecollection at time interval from 8 to 9h AM and sample pretreatment in 0,6% colchicine solution for 30 minutes allow obtaining the highest accumulation of mitotic cells in metaphase. Root tip fixation in amixture of of 1 part glacial acetic acid and 2 parts of absolute ethanol, and sample incubation in 0,6% cellulase solution provide good chromosome spreading and high quality plant chromosome preparations.
References
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Published
Nov 30, 2016
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How to Cite
Nguyen Minh Ly*, Le Van Kiem, Ngo Thi Hong Van, Le Thi Mai. “Aplication of Method ‘StreamDrop’ for Chromosome Preparation in Allium Ascalonicum L”. The University of Danang - Journal of Science and Technology, vol. 11, no. 108.1, Nov. 2016, pp. 86-89, https://jst-ud.vn/jst-ud/article/view/1729.
