RESEARCHING CHARACTERISTICS AND EXPRESSIONS OF BETA-CYANOALANINE SYNTHASE GENES IN THE CASSAVA (MANIHOT ESCULENTA CRANTZ) VIA BIOINFORMATIC METHODS

Abstract: 110 | PDF: 50

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Author

  • Cao Phi Bang*

Keywords:

-cyanoalanine synthase
cassava
gene expression
gene structure
phylogenetic tree
bioinfomatic

Abstract

-cyanoalanine synthase (CAS) is a key enzyme in the detoxification of HCN in high-rank plants. In this study, we identified two genes encoded for CAS in the cassava genome by employing bioinformatics methods. The cassava CASs were highly similar to the CASs in other known plant species. Both cassava CAS genes had 9 introns in total. The predicted polypeptides encoded by cassava CAS genes had a mass of 40 kDa, a high pI value, and carried mitochondria localization signals. The important enzyme-like amino acids in PLP-bound sites or the reaction centre, were highly conservative in both CASs of the cassava. The phylogenetic tree analysis showed that the CASs of the cassava were very close to their orthologs in rubber trees. The survey results showed that both CAS genes of the cassava were encoded in its vegetative tissues under normal conditions. In addition, the MesCAS1 gene encoded in the vegetative tissues of plants under water shortage conditions.

References

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Published

Jul 31, 2015
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How to Cite
Cao Phi Bang*. “RESEARCHING CHARACTERISTICS AND EXPRESSIONS OF BETA-CYANOALANINE SYNTHASE GENES IN THE CASSAVA (MANIHOT ESCULENTA CRANTZ) VIA BIOINFORMATIC METHODS”. The University of Danang - Journal of Science and Technology, vol. 7, no. 92, July 2015, pp. 76-79, https://jst-ud.vn/jst-ud/article/view/2974.

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