The Effect of Culture Medium Components on Production of Extracellular Polysaccharide (EPS) with Antioxidant Activity in Ophiocordyceps Sinensis Fungus
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Keywords:
Cordyceps sinensis
exopolysaccharide
ABTS (2,2'- azino - bis (3 – ethylbenzothiazoline - 6 - sulphonic acid))
surface liquid cultures
antioxidant activity
Abstract
Currently, Cordyceps sinensis (Dong Trung Ha Thao) receives much interest from scientific community because of many bioactive compounds such as: cordycepin, nucleoside, ergosterol, polysaccharide … C. sinensis was successfully artificially cultured in liquid medium in Vietnam. However, after incubation, only mycelial biomass, rather than culture fluid, was produced and studied. Many studies indicate that the culture fluid of C. sinensis contains bioactive EPS. In this study, We investigate the EPS of C. sinensis in the liquid of static culture by supplementing with peptone, yeast or silkworms compared to the control culture. Then, We assess the antioxidant activity of EPS from the culture fluid by ABTS free radical method. Results indicate that C. sinensis has the ability to adapt to and develop well in these media as well as stimulate the EPS production. Particularly, the medium within peptone and yeast gives the highest biomass and EPS of 19.3g / l and 2.5g / l. ABTS activity of EPS from it also increases significantly in 40 culture days.
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Published
May 31, 2017
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How to Cite
LE THI THUY HANG*, Tran Dang My Ngan, Nguyen Thi Kim Hau, Nguyen Thanh Son, Dinh Minh Hiep, Nguyen Tien Thang, Nguyen Thi Kim Oanh. “The Effect of Culture Medium Components on Production of Extracellular Polysaccharide (EPS) With Antioxidant Activity in Ophiocordyceps Sinensis Fungus”. The University of Danang - Journal of Science and Technology, vol. 5, no. 114.1, May 2017, pp. 99-103, https://jst-ud.vn/jst-ud/article/view/3142.
