Ảnh hưởng của nhiệt độ chiết xuất đến hoạt tính chống oxy hóa của polysacharides từ lá sâm đá (Myxopyrum smilacifolium)

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Author

  • Le Trung Hieu
    University of Sciences, Hue University, Thua Thien Hue, Vietnam
    Le Lam Son
    University of Sciences, Hue University, Thua Thien Hue, Vietnam
    Nguyen Minh Nhung
    University of Sciences, Hue University, Thua Thien Hue, Vietnam
    Ho Xuan Anh Vu
    University of Sciences, Hue University, Thua Thien Hue, Vietnam
    Nguyen Quang Man
    University of Medicine and Pharmacy, Hue University, Thua Thien Hue, Vietnam
    Tran Thanh Minh
    University of Sciences, Hue University, Thua Thien Hue, Vietnam
    Nguyen Viet Thang
    University of Sciences, Hue University, Thua Thien Hue, Vietnam
    Nguyen Thi Nhu
    University of Sciences, Hue University, Thua Thien Hue, Vietnam
    Nguyen Thi Hong Hanh
    University of Sciences, Hue University, Thua Thien Hue, Vietnam
    Nguyen Hoang Luong Ngoc
    University of Sciences, Hue University; Ho Chi Minh City University of Industry and Trade, Ho Chi Minh, Vietnam
    Tran Thi Van Thi
    University of Sciences, Hue University, Thua Thien Hue, Vietnam

Từ khóa:

ABTS
khả năng chống oxy hoá
DPPH
Myxopyrum smilacifolium
polysaccharide

Tóm tắt

Mục tiêu của bài báo này là nghiên cứu ảnh hưởng của nhiệt độ chiết đến tiềm năng chống oxy hóa in vitro của polysaccharide thu được từ lá Sâm đá (Myxopyrum smilacifolium). Kết quả cho thấy, ở nhiệt độ chiết là 90°C thì hoạt tính chống oxy hóa của polysaccharide là cao nhất. Phân tích quang phổ hồng ngoại biến đổi Fourier cho thấy, PS-T90 thể hiện các nhóm hấp thụ đặc trưng thường thấy trong các cấu trúc polysaccharide. Trọng lượng phân tử trung bình của polysaccharide được chiết xuất là khoảng 9,30×105 Da. Tổng hàm lượng chống oxy hóa quy tương đương của PS-T90 được xác định là 0,2646 ± 0,0007 mg GA/g hoặc 0,1725 ± 0,0007 mg AS/g. Hơn nữa, các giá trị IC50 trong các mô hình bắt gốc tự do DPPH và ABTS của PS-T90 lần lượt là 1,04 mg/mL và 3,37 mg/mL. Hoạt tính sinh học nổi bật như vậy có thể thúc đẩy việc sử dụng PS-T90 như một nguồn đầy hứa hẹn để phát triển chất chống oxy hóa.

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Nov 30, 2023
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Le Trung Hieu, Le Lam Son, Nguyen Minh Nhung, Ho Xuan Anh Vu, Nguyen Quang Man, Tran Thanh Minh, Nguyen Viet Thang, Nguyen Thi Nhu, Nguyen Thi Hong Hanh, Nguyen Hoang Luong Ngoc, và Tran Thi Van Thi. “Ảnh hưởng của nhiệt độ chiết xuất đến hoạt tính chống Oxy hóa của Polysacharides từ Lá sâm đá (Myxopyrum Smilacifolium)”. Tạp Chí Khoa học Và Công nghệ - Đại học Đà Nẵng, vol 21, số p.h 11.2, Tháng Mười-Một 2023, tr 108-12, https://jst-ud.vn/jst-ud/article/view/8701.

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