Explore the antibiofilm ability of Ampelopsis cantoniensis extract against Staphylococcus aureus via experimental and computational approaches
Tóm tắt: 131
| 
PDF: 76 
##plugins.themes.academic_pro.article.main##
Author
Từ khóa:
Ampelopsis cantoniensis
Staphylococcus aureus
Anti-biofilm activity
Virtual screening
Sortase A
Tóm tắt
Our study aimed to evaluate the anti-biofilm activity of the crude ethanolic extract of Ampelopsis cantoniensis against Staphylococcus aureus (S. aureus) isolated from clinical specimens. Biofilm inhibition was assessed at 6, 16, and 24 h using two concentrations of the extract: 0.4 mg/ml (MIC) and 0.2 mg/ml (½ MIC). At the MIC concentration, the extract achieved a biofilm inhibition rate of 69.91% after 24 h. To explore the bioactive constituents responsible for this effect, virtual screening was conducted, identifying 3’,5’,5,7-tetrahydroxyflavanone as a promising anti-biofilm compound. Molecular docking analysis revealed that this compound interacts with Sortase A (SrtA) and SarA, two essential regulatory proteins involved in S. aureus biofilm formation. These findings suggest that A. cantoniensis, particularly its constituent 3′,5′,5,7-tetrahydroxyflavanone, can be potential for the development of novel anti-biofilm agents targeting S. aureus.
Tài liệu tham khảo
-
[1] S. Y. C. Tong, J. S. Davis, E. Eichenberger, T. L. Holland, and V. G. Fowler, “Staphylococcus aureus Infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management”, Clin Microbiol Rev, vol. 28, no. 3, pp. 603–661, Jul. 2015.
[2] K. M. Craft, J. M. Nguyen, L. J. Berg, and S. D. Townsend, “Methicillin-resistant Staphylococcus aureus (MRSA): antibiotic-resistance and the biofilm phenotype”, Medchemcomm, vol. 10, no. 8, pp. 1231–1241, 2019.
[3] L. Karygianni, Z. Ren, H. Koo, and T. Thurnheer, “Biofilm Matrixome: Extracellular Components in Structured Microbial Communities”, Trends Microbiol, vol. 28, no. 8, pp. 668–681, Aug. 2020.
[4] H. C. Flemming, J. Wingender, U. Szewzyk, P. Steinberg, S. A. Rice, and S. Kjelleberg, “Biofilms: an emergent form of bacterial life”, Nature Reviews Microbiology, vol. 14, no. 9, pp. 563–575, Aug. 2016.
[5] A. H. Bhat, M. T. Nguyen, A. Das, and H. Ton-That, “Anchoring surface proteins to the bacterial cell wall by sortase enzymes: How it started and what we know now”, Curr Opin Microbiol, vol. 60, p. 73, Apr. 2021.
[6] L. Li et al., “The Global Regulon sarA Regulates β-Lactam Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus In Vitro and in Endovascular Infections”, The Journal Infectious Diseases, vol. 214, no. 9, pp. 1421–1429, Nov. 2016.
[7] X.-N. Xiao, F. Wang, Y.-T. Yuan, J. Liu, Y.-Z. Liu, and X. Yi, “Antibacterial Activity and Mode of Action of Dihydromyricetin from Ampelopsis grossedentata Leaves against Food-Borne Bacteria”, Molecules, vol. 24, no. 15, p. 2831, Aug. 2019.
[8] N. V. Thu et al., “Anti-inflammatory Compounds from Ampelopsis cantoniensis”, Nat Prod Commun, vol. 10, no. 3, pp. 383–385, 2015.
[9] N. T. B. Van et al., “In vitro antibacterial activity of Ampelopsis cantoniensis extracts cultivated at Danang against clinically isolated Staphylococcus aureus”, TNU Journal of Science and Technology, vol. 227, no. 10, pp. 235–242, Jul. 2022.
[10] N. T. B. Van et al., “Investigating the antibacterial mechanism of Ampelopsis cantoniensis extracts against methicillin-resistant Staphylococcus aureus via in vitro and in silico analysis”, J Biomol Struct Dyn, vol. 41, no. 23, pp. 14080–14091, Dec. 2023.
[11] S. A. Burt, V. T. A. Ojo-Fakunle, J. Woertman, and E. J. A. Veldhuizen, “The Natural Antimicrobial Carvacrol Inhibits Quorum Sensing in Chromobacterium violaceum and Reduces Bacterial Biofilm Formation at Sub-Lethal Concentrations”, PLoS One, vol. 9, no. 4, p. e93414, Apr. 2014.
[12] P. Liu, X. Kang, X. Chen, X. Luo, C. Li, and G. Wang, “Quercetin targets SarA of methicillin-resistant Staphylococcus aureus to mitigate biofilm formation”, Microbiol Spectr, vol. 12, no. 1, Jan. 2024.
[13] O. I. Zhuravleva et al., “Anthraquinone Derivatives and Other Aromatic Compounds from Marine Fungus Asteromyces cruciatus KMM 4696 and Their Effects against Staphylococcus aureus”, Mar Drugs, vol. 21, no. 8, Aug. 2023.
[14] M. F. Akter et al., “Antibacterial and antibiofilm activity of Eryngium foetidum essential oil”, Khulna University Studies, vol. 21, no. 1, pp. 195–206, Jun. 2024.
[15] X. Niu et al., “Molecular Modelling reveals the inhibition mechanism and structure–activity relationship of curcumin and its analogues to Staphylococcal aureus Sortase A”, J Biomol Struct Dyn, vol. 37, no. 5, pp. 1220–1230, Mar. 2019.
[16] H. Lade et al., “Biofilm Formation by Staphylococcus aureus Clinical Isolates is Differentially Affected by Glucose and Sodium Chloride Supplemented Culture Media”, Journal of Clinical Medicine, vol. 8, no. 11, p. 1853, Nov. 2019.
[17] Y. Lim, M. Jana, T. T. Luong, and C. Y. Lee, “Control of Glucose- and NaCl-Induced Biofilm Formation by rbf in Staphylococcus aureus”, J Bacteriol, vol. 186, no. 3, pp. 722–729, Feb. 2004.
[18] D. Saeloh and M. Visutthi, “Efficacy of Thai Plant Extracts for Antibacterial and Anti-Biofilm Activities against Pathogenic Bacteria”, Antibiotics, vol. 10, no. 12, p. 1470, Dec. 2021.
[19] F. Blando, R. Russo, C. Negro, L. De Bellis, and S. Frassinetti, “Antimicrobial and Antibiofilm Activity against Staphylococcus aureus of Opuntia ficus-indica (L.) Mill. Cladode Polyphenolic Extracts”, Antioxidants, vol. 8, no. 5, p. 117, May 2019.
[20] Y. Mu, H. Zeng, and W. Chen, “Quercetin Inhibits Biofilm Formation by Decreasing the Production of EPS and Altering the Composition of EPS in Staphylococcus epidermidis”, Front Microbiol, vol. 12, p. 631058, Mar. 2021.
[21] Y. Sun et al., “Luteolin Inhibits the Biofilm Formation and Cytotoxicity of Methicillin-Resistant Staphylococcus aureus via Decreasing Bacterial Toxin Synthesis”, Evid Based Complement Alternat Med, vol. 2022, p. 4476339, 2022.
[22] Y. Mao, P. Liu, H. Chen, Y. Wang, C. Li, and Q. Wang, “Baicalein Inhibits the Staphylococcus aureus Biofilm and the LuxS/AI-2 System in vitro”, Infect Drug Resist, vol. 16, p. 2861, 2023.
[23] P. Liu, X. Kang, X. Chen, X. Luo, C. Li, and G. Wang, “Quercetin targets SarA of methicillin-resistant Staphylococcus aureus to mitigate biofilm formation”, Microbiol Spectr, vol. 12, no. 1, pp. e02722-23, Jan. 2023.
[24] Q. Yuan et al., “Luteolin attenuates the pathogenesis of Staphylococcus aureus by interfering with the agr system”, Microb Pathog, vol. 165, p. 105496, Apr. 2022.
[25] F. Esposito et al., “Hypericum hircinum L. components as new single-molecule inhibitors of both HIV-1 reverse transcriptase-associated DNA polymerase and ribonuclease H activities”, Pathog Dis, vol. 68, no. 3, pp. 116–124, Aug. 2013.
[26] W. W. Ma, Z. Q. Huang, Y. Zhang, K. Liu, D. Z. Li, and Q. Liu, “Interaction between inflammation and biofilm infection and advances in targeted biofilm therapy strategies”, Microbiol Res, vol. 298, p. 128199, Sep. 2025.
[27] N. Aziz, M. Y. Kim, and J. Y. Cho, “Anti-inflammatory effects of luteolin: A review of in vitro, in vivo, and in silico studies”, J Ethnopharmacol, vol. 225, pp. 342–358, Oct. 2018.
[28] X. Hao, Y. Bai, W. Li, and M. X. Zhang, “Phloretin attenuates inflammation induced by subarachnoid hemorrhage through regulation of the TLR2/MyD88/NF-kB pathway”, Sci Rep, vol. 14, no. 1, p. 26583, Dec. 2024.
Xem thêm
##plugins.themes.academic_pro.article.sidebar##
Đã Xuất bản
Sep 30, 2025
Download
Cách trích dẫn
Bich, V. N. T., Y. N. H. Thien, C. T. Bui, T. P. T. Doan, và P. T. V. Pham. “Explore the Antibiofilm Ability of Ampelopsis Cantoniensis Extract Against Staphylococcus Aureus via Experimental and Computational Approaches”. Tạp Chí Khoa học Và Công nghệ - Đại học Đà Nẵng, vol 23, số p.h 9C, Tháng Chín 2025, tr 37-42, doi:10.31130/ud-jst.2025.23(9C).532E.
