In-vitro antibacterial activity of the fractions from Cleistocalyx operculatus (Roxb.) Merr. et Perry against Staphylococcus aureus
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Từ khóa:
Staphylococcus aureus
Cleistocalyx operculatus
antimicrobial activity
flavonoid
Tóm tắt
The prevalence of Gram-positive Staphylococcus aureus in infectious diseases is increasing in Vietnam for recent years. Therefore, novel class of antibiotic or active compounds is indispensable to treat this common worldwide pathogen. In the present study, we investigated the antibacterial activities of Cleistocalyx operculatus (Roxb.) Merr. et Perry leaves collected in Quang Nam. The results showed that ethanolic crude extract and three fractions partitioned in different polar solvents suppressed Staphylococcus aureus ATCC 6538. The ethyl acetate and n-hexan fractions exhibited a significant inhibitory effect, with minimum inhibitory concentrations (MIC) values of 0.1 mg/ml and 0.2 mg/ml, respectively. And flavonoids was possibly the active substances responsible for the antimicrobial activity of this medical plant. These initial findings are the premise of further studies for the treatment of infection. It also provides the scientific evidences for the traditional utilization of Cleistocalyx operculatus (Roxb.) Merr. et Perry leaves.
Tài liệu tham khảo
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[1] Y. C. Tong, J. S. Davis, E. Eichenberger, T. L. Holland, and V. G. Fowler, “Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management”, Clin. Microbiol. Rev., vol. 28, no. 3, pp. 603–661, 2015.
[2] Zheng et al., “Antibacterial and Anti-biofilm Efficacy of Chinese Dragon’s Blood Against Staphylococcus aureus Isolated From Infected Wounds”, Front. Microbiol., vol. 12, 2021.
[3] Van Nam et al., “Antibiotic Resistance Profile and Methicillin-Resistant Encoding Genes of Staphylococcus aureus Strains Isolated from Bloodstream Infection Patients in Northern Vietnam”, vol. 7, no. 24, pp. 4406–4410, 2019.
[4] M. E. Etame et al., “Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract”, BMC Complement. Altern. Med., vol. 19, no. 1, pp. 1–7, 2019.
[5] Manilal et al., “In vitro antibacterial activity of medicinal plants against biofilm-forming methicillin-resistant Staphylococcus aureus: efficacy of Moringa stenopetala and Rosmarinus officinalis extracts”, Heliyon, vol. 6, no. 1, p. e03303, 2020.
[6] Tozar et al., “Anti-staphylococcal activity and mode of action of thioridazine photoproducts”, Sci. Rep., vol. 10, no. 1, pp. 1–12, 2020.
[7] Pancreatic, C. Cell, H. N. Tuan, B. H. Minh, P. T. Tran, and J. H. Lee, “The Effects of 2’,4’-Dihydroxy-6’-methoxy-3’,5’- dimethylchalcone from Cleistocalyx operculatus Buds on Human Pancreatic Cancer Cell Lines”, Molecules, vol. 24, no. 2538, pp. 1–11, 2019.
[8] T. Mai, N. Fumie, and N. Van Chuyen, “Antioxidant activities and hypolipidemic effects of an aqueous extract from flower buds of Cleistocalyx operculatus (Roxb.) Merr. and Perry”, J. Food Biochem., vol. 33, no. 6, pp. 790–807, 2009.
[9] L. Tran et al., “Protective effects of extract of Cleistocalyx operculatus flower buds and its isolated major constituent against LPS-induced endotoxic shock by activating the Nrf2/HO-1 pathway”, Food Chem. Toxicol., vol. 129, pp. 125–137, 2019.
[10] Thi, M. Nguyen, N. Schultze, C. Boger, Z. Alresley, and A. Bolhuis, “Anticaries and antimicrobial activities of methanolic extract from leaves of Cleistocalyx operculatus L.”, Asian Pac. J. Trop. Biomed., vol. 7, no. 1, pp. 43–48, 2017.
[11] B. Tran, N. T. T. Le, and S. M. Dam, “Potential use of essential oil isolated from cleistocalyx operculatus leaves as a topical dermatological agent for treatment of burn wound”, Dermatol. Res. Pract., vol. 2018, pp1-8, 2018.
[12] T. B. Van, T. T. T. Hien, and P. T. T. Anh, “To investigate antimicrobial activity of crude extract of cleistocalyxoperculatus leaves and initially make souble powder”, The University of Danang - Journal of Science and Technology, vol. 19, no. 5.2, pp. 44–47, 2021.
[13] Thouri, H. Chahdoura, A. E. Arem, A. O. Hichri, R. B. Hassin, and L. Achour, “Effect of solvents extraction on phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti)”, BMC Complement. Altern. Med., vol. 17, no. 1, pp. 1–10, 2017.
[14] I. Dirar, D. H. M. Alsaadi, M. Wada, M. A. Mohamed, T. Watanabe, and H. P. Devkota, “Effects of extraction solvents on total phenolic and flavonoid contents and biological activities of extracts from Sudanese medicinal plants”, South African J. Bot., vol. 120, pp. 261–267, 2019.
[15] Balouiri, M. Sadiki, and S. K. Ibnsouda, “Methods for in vitro evaluating antimicrobial activity: A review”, J. Pharm. Anal., vol. 6, no. 2, pp. 71–79, 2016.
[16] H. Teh, W. A. Nazni, A. H. Nurulhusna, A. Norazah, and H. L. Lee, “Determination of antibacterial activity and minimum inhibitory concentration of larval extract of fly via resazurin-based turbidometric assay”, BMC Microbiol., vol. 17, no. 1, pp. 1–8, 2017.
[17] Mogana, A. Adhikari, M. N. Tzar, R. Ramliza, and C. Wiart, “Antibacterial activities of the extracts, fractions and isolated compounds from canarium patentinervium miq. Against bacterial clinical isolates”, BMC Complement. Med. Ther., vol. 20, no. 1, pp. 1–11, 2020.
[18] C. Chang, M. H. Yang, H. M. Wen, and J. C. Chern, “Estimation of total flavonoid content in propolis by two complementary colometric methods”, J. Food Drug Anal., vol. 10, no. 3, pp. 178–182, 2002.
[19] S. Min, T. D. Cuong, J. S. Lee, M. H. Woo, and T. M. Hung, “Flavonoids from cleistocalyx operculatus buds and their cytotoxic activity”, Bull. Korean Chem. Soc., vol. 31, no. 8, pp. 2392–2394, 2010.
[20] T. Dung, J. M. Kim, and S. C. Kang, “Chemical composition, antimicrobial and antioxidant activities of the essential oil and the ethanol extract of Cleistocalyx operculatus (Roxb.) Merr and Perry buds”, Food Chem. Toxicol., vol. 46, no. 12, pp. 3632–3639, 2008.
[21] Elshikh et al., “Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants”, Biotechnol. Lett., vol. 38, no. 6, pp. 1015–1019, 2016.
[22] C. O. Silva et al., “Which approach is more effective in the selection of plants with antimicrobial activity?”, Evidence-based Complement. Altern. Med., vol. 2013, 2013.
[23] Nawaz, M. A. Shad, N. Rehman, H. Andaleeb, and N. Ullah, “Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds”, Brazilian J. Pharm. Sci., vol. 56, 2020.
[24] T. Thuy, B. T. Thuong, B. T. My, D. T. H. Linh, P. H. Minh, and B. T. Tung, “Effect of Cleistocalyx operculatus and Morus alba extracts on DSS-induced acute colitis in mice”, Curr. Top. Pharmacol., vol. 25, pp. 35–41, 2021.
[25] S. Dosoky, S. K. Pokharel, W. N. Setzer, and C. N. William Setzer, “Leaf essential oil composition, antimicrobial and cytotoxic activities of Cleistocalyx operculatus from Hetauda, Nepal”, Am. J. Essent. Oils Nat. Prod., vol. 3, no. 1, pp. 34–37, 2015.
[26] T. Reetz and G. König, “n-Butanol: An Ecologically and Economically Viable Extraction Solvent for Isolating Polar Products from Aqueous Solutions”, European J. Org. Chem., vol. 2021, no. 46, pp. 6224–6228, 2021.
[27] G. Jung, J. Y. Jeong, S. H. Yum, and Y. J. Hwang, “Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant Staphylococcus aureus”, Molecules, vol. 27, no. 22, pp. 1–13, 2022.
[28] O. Chaves et al., “Extraction of Flavonoids From Natural Sources Using Modern Techniques”, Front. Chem., vol. 8, 2020.
[29] Biharee, A. Sharma, A. Kumar, and V. Jaitak, “Fitoterapia Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance”, Fitoterapia, vol. 146, p. 104720, 2020.
[30] T. T. Dao et al., “C-methylated flavonoids from cleistocalyx operculatus and their inhibitory effects on novel influenza a (H1N1) neuraminidase”, J. Nat. Prod., vol. 73, no. 10, pp. 1636–1642, 2010.
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Jun 30, 2023
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Thai Bich, V. N., T. D. Thi Thu, T. H. Tran, và P. T. Vinh Phu. “In-Vitro Antibacterial Activity of the Fractions from Cleistocalyx Operculatus (Roxb.) Merr. Et Perry Against Staphylococcus Aureus”. Tạp Chí Khoa học Và Công nghệ - Đại học Đà Nẵng, vol 21, số p.h 6.1, Tháng Sáu 2023, tr 56-60, doi:10.31130/ud-jst.2023.056E.
