Methodology for quick analysis of curcumin, demethoxycurcumin và bisdemethoxycurcumin from turmeric by agilent 1260 HPLC system

Abstract: 775 | PDF: 1895

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Author

  • Phi-Hung Nguyen
    Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Vietnam
    Anh-Tuan Nguyen
    Hanoi National University of Education, Vietnam
    Thi-Tu Dinh
    Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Vietnam
    Dao-Cuong To
    Nano Institute (PHENA), Phenikaa University, Vietnam
    Thi-Phuong Hoang
    Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), Vietnam
    Dang Ngoc Quang
    Hanoi National University of Education, Vietnam
    Thi-Thuy Do
    Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Vietnam
    Thi-Ha Vu
    Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Vietnam
    Thi-Thuc Bui
    Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Vietnam
    Thi-Thu-Ha Trinh
    Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Vietnam

Keywords:

Curcumin
high performance liquid chromatography (HPLC)
Demethoxycurcumin
Bisdemethoxycurcumin
Turmeric
Curcuma longa

Abstract

A high performance liquid chromatography (HPLC) method has been built for qualitative and quantitative analysis of curcumin (1), demethoxycurcumin (2) and bisdemethoxycurcumin (3) with specificity and high reliability. Experimental results show that the correlation coefficient R2 of compounds (1–3) is greater than 0.9999, the average recovery in each sample is greater than 97% (with 98.60; 98.24, and 100.49% for compounds 1–3, respectively), the RSD values are all less than 1% (0.629 to 0.750 for retention time; 0.428 to 0.756 for peak area). Meanwhile, Limit of Detection (LOD) and Limit of Quantification (LOQ) values of compounds
1–3 are low with values in the range of 0.04 – 1.05 μg/mL and 0.35 – 2.50 μg/mL, respectively, indicating the method is built with high sensitivity according to Association of Official Analytical Chemists (AOAC) standard. The method was applied to analyze the content of three curcumins (1–3) in a number of different turmeric samples collected in Vietnam.

References

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Published

May 31, 2023
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How to Cite
Nguyễn Phi Hùng, Nguyễn Anh Tuấn, Đinh Thị Tú, Tô Đạo Cường, Hoàng Thị Phương, Đặng Ngọc Quang, Đỗ Thị Thúy, Vũ Thị Hà, Bùi Thị Thực, and Trịnh Thị Thu Hà. “Methodology for Quick Analysis of Curcumin, Demethoxycurcumin Và Bisdemethoxycurcumin from Turmeric by Agilent 1260 HPLC System”. The University of Danang - Journal of Science and Technology, vol. 21, no. 5, May 2023, pp. 103-9, https://jst-ud.vn/jst-ud/article/view/8335.

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