Chitosan/ biphasic calcium phosphate nanofibrous scaffolds for bone tissue engineering

https://doi.org/10.31130/jst-ud2020-113E

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Author

  • Truong Le Bich Tram

Từ khóa:

nanofibers
chitosan/ biphasic
osteogenic cells
photocrosslinking
the tissue engineering of bones

Tóm tắt

In this article, chitosan/biphasic calcium phosphate (CS/BCP)nanofibers were prepared by electrospinning. From the culture of osteogenic cells, the biocompatibility of CS/BCP nanofibrous substrates was identified and increased by the photocrosslinking. The enhancement in cell attachment and proliferation was caused by the improvement in nanofibers’ mechanical properties. The biocompatibility to osteoblasts was also promoted with the content of BCP. The osteogenic differentiation in early, middle and late stage was encouraged by the addition of BCP on nanofibrous substrates. The CS/BCP nanofibers were highly specific to osteogenic cells, revealed by difficulties in the growth of non-osteogenic cells on this composite nanofibrous scaffold. The novel nanofibrous scaffolds showed great potential in the tissue engineering of bones.

Tài liệu tham khảo

    [1] Cenni E., Pizzoferrato A., Ciapetti G., Granchi D., Savarino L., Stea S., “Inflammatory response to metals and ceramics”. Integrated Biomaterials Science, 2002. p. 735–791.
    [2] Wagh A., “Chemically Bonded Phosphate Ceramics: Twenty-First Century Materials with Diverse Applications”. Elsevier Science, 2004.
    [3] Veis A., “Phosphoproteins from teeth and bone”. Ciba Found Symp, 1998. 136, p. 161-177.
    [4] Songtao Shi, Martin Kirk, Arnold J. Kahn, “The role of type I collagen in the regulation of the osteoblast phenotype”. Journal of Bone and Mineral Research, 1996. 11(8), p. 1139-1145.
    [5] Boskey AL., Posner AS., “Bone structure, composition, and mineralization”. Orthop Clin North Am, 1984. 15(597-612).
    [6] Schiffman JD., “Schauer CL., Cross-linking chitosan nanofibers”. Biomacromolecules, 2007. 8, p. 594– 601.
    [7] Mi FL., Tan YC., Liang HC., Huang RN., Sung HW., “In vitro evaluation of a chitosan membrane cross-linked with genipin”. J. Biomater. Sci. Polym., 2001. 12, p. 835– 850.
    [8] Eric R. Schauer Welsh, Santos CL., John PP, Ronald R., “In Situ Cross-Linking of Alternating Polyelectrolyte Multilayer Films”. Langmuir, 2004. 20(5), p. 1807-1811.
    [9] Wan Ngah WS., Endud CS., Mayanar R., “Removal of copper(II) ions from aqueous solution onto chitosan and cross-linked chitosan beads”. Reactive and Functional Polymers, 2002. 50(2), p. 181-190.
    [10] Zhang Sisson K., Farach-Carson C., Chase MC., Rabolt DB., “Evaluation of cross-linking methods for electrospun gelatin on cell growth and viability”. Biomacromolecules, 2009. 10(7), p. 1675-1680.
    [11] Yu Jin, Dongzhi Yang, Yingshan Zhou, Guiping Ma, “Jun Nie, Photocrosslinked electrospun chitosan-based biocompatible nanofibers”. Journal of Applied Polymer Science, 2008. 109(5), p. 3337-3343.
    [12] Aduba DJ., “Bachelor of science”. Master of Science, Biomedical Engineering, 2008.
    [13] HaiYan Yin, Jong Hyun Ko, Jeongho An, Dong June Chung, Ji-Heung Kim, Soo Bok Lee, Do Gi Pyun, “Characterization of cross-linked gelatin nanofibers through electrospinning”. Macromolecular Research, 2010. 18(2), p. 137-143.
    [14] Jelena Rnjak Kovacina, Steven G. Wise, Zhe Li, Peter KM. Maitz, Cara J. Young, Yiwei Wang, “Anthony S. Weiss, Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering”. Biomaterials, 2011. 32(28), p. 6729-6736.
    [15] Xuan Cai, Hua Tong, Xinyu Shen, Weixuan Chen, Juan Yan, Jiming Hu, “Preparation and characterization of homogeneous chitosan–polylactic acid/hydroxyapatite nanocomposite for bone tissue engineering and evaluation of its mechanical properties”. Acta Biomaterialia, 2009. 5(7), p. 2693-2703.
    [16] Hae-Won Kim, Jonathan C. Knowles, Hyoun-Ee Kim, “Hydroxyapatite and gelatin composite foams processed via novel freeze-drying and crosslinking for use as temporary hard tissue scaffolds”. Journal of Biomedical Materials Research Part A, 2005. 72A(2), p. 136-145.
    [17] Hae-Won Kim, Hyoun-Ee Kim, Vehid Salih, “Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin–hydroxyapatite for tissue engineering scaffolds”. Biomaterials, 2005. 26(25), p. 5221-5230.
    [18] Frauchiger V., Baxter LC., “Textor M., Gwynn I., Richards RG., Fibroblast and osteoblast adhesion and morphology on calcium phospha te surfaces”. eCM Journal, 2002. 4, p. 1-17.
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Jun 30, 2020
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Cách trích dẫn
Truong Le Bich Tram. “Chitosan/ Biphasic Calcium Phosphate Nanofibrous Scaffolds for Bone Tissue Engineering”. Tạp Chí Khoa học Và Công nghệ - Đại học Đà Nẵng, vol 18, số p.h 6, Tháng Sáu 2020, tr 11-17, doi:10.31130/jst-ud2020-113E.

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